Method Description

TT-seq is a variant of 4-thiouridine (4sU) sequencing.  This approach labels newly synthesized RNA by metabolic incorporation of 4sU in live cells.  Analysis of this RNA can provide information on RNA synthesis, co-transcriptional processing, and degradation (depending on experimental design).

Service Description

Users submit total RNA isolated from cells pulsed with 4sU.  The core fragments the total RNA, enriches 4sU labeled fragments, constructs Illumina compatible RNA-seq libraries, and performs preliminary data analysis.

The amount of material required depends on duration of metabolic labeling and the cell type being labeled and can range from 0.5-2% of total RNA.  We request that users provide 50-100ug of total RNA but please contact us for discussion of the amount of labeled RNA required to construct libraries for your project.

Further Reading