Welcome to the Nascent Transcriptomics Core

The mission of the Nascent Transcriptomics Core (NTC) is to offer the community a resource for the analysis of nascent transcription, providing direct insights into gene regulation and enabling highly-sensitive identification of enhancers and long non-coding RNAs.

We offer consultation on experimental design as well as library construction services for Start-seq, PRO-seq and metabolically labeled RNA-seq (e.g. TT-seq). In addition, the NTC looks forward to expanding these offerings as we develop and optimize new RNA sequencing methods in partnership with the HMS community.

Analysis of nascent RNA, meaning RNA being actively synthesized by RNA polymerase, reveals the process of transcript elongation. This directly exposes alterations in patterns of gene expression and identifies unstable splicing intermediates or non-coding RNA transcripts that are challenging to detect by total RNA sequencing.  Some applications of nascent RNA analysis include:

  • Pinpoint enhancers by sequencing enhancer RNAs
  • Define long non-coding RNAs expressed in a specific cell type or condition
  • Identify sites of transcriptional initiation or pausing at single-nucleotide resolution
  • Observe unstable RNA processing intermediates, and calculate rates of RNA processing or decay

If you are interested in our services, or want to find out how these methods could help your research, please fill out the consultation request from on the Project Application page or contact us directly.

The Nascent Transcriptomics Core is part of the HMS Epigenetics & Gene Dynamics Initiative