Welcome to the Nascent Transcriptomics Core

NTC Operational update

We are pleased to announce the Nascent Transcriptomics Core is reopening.  As of Monday, June 22nd we will resume processing user samples and are able to receive new samples.  Please see our sample submission guide for instructions.  For users with projects pending prior to our shutdown, we'll be in touch with you via email but don't hesitate to get in touch before then.  For users interested in starting new projects, we remain available for consultations.

Please note that the core has moved to allow for safe social distancing during this challenging time.  We are now located in the nearby C-building, Room 302-A across the hall from the CMI core.  Our new address is:

Nascent Transcriptomics Core
Harvard Medical School
240 Longwood Ave.
C-Building, Room 302-A
Boston, MA 02115
 

Overview

The mission of the Nascent Transcriptomics Core (NTC) is to offer the community a resource for the analysis of nascent transcription, providing direct insights into gene regulation and enabling highly-sensitive identification of enhancers and long non-coding RNAs.

We offer consultation on experimental design as well as library construction services for Start-seq, PRO-seq and metabolically labeled RNA-seq (e.g. TT-seq). In addition, the NTC looks forward to expanding these offerings as we develop and optimize new RNA sequencing methods in partnership with the HMS community.

Analysis of nascent RNA, meaning RNA being actively synthesized by RNA polymerase, reveals the process of transcript elongation. This directly exposes alterations in patterns of gene expression and identifies unstable splicing intermediates or non-coding RNA transcripts that are challenging to detect by total RNA sequencing.  Some applications of nascent RNA analysis include:

  • Pinpoint enhancers by sequencing enhancer RNAs
  • Define long non-coding RNAs expressed in a specific cell type or condition
  • Identify sites of transcriptional initiation or pausing at single-nucleotide resolution
  • Observe unstable RNA processing intermediates, and calculate rates of RNA processing or decay

If you are interested in our services, or want to find out how these methods could help your research, please fill out the consultation request from on the Project Application page or contact us directly.

The Nascent Transcriptomics Core is part of the HMS Epigenetics & Gene Dynamics Initiative