Sample Guide
Currently the core offers two different nascent RNA library construction services: PRO-seq and TT-seq. Please see the links in the side-bar for detailed information about each of them and the table below for an overview of sample requirements.
| Method | Minimum # cells | Requested # of cells | Cell condition | Spike-in available | Spike-in required |
|---|---|---|---|---|---|
PRO-seq | 1 million | 1-3 million | permeabilized frozen cells | yes | yes |
TT-seq | 5-8 million * | 5-8 million* | 4sU labeled cells in Trizol | yes | yes |
* Number of cells needed for TT-seq depends on cell type and labeling time. We request 100ug of total RNA.
If you are interested in differential expression analysis, we recommend preparing a minimum of biological duplicates of your samples. For other applications, more or fewer replicates may be appropriate. We encourage users—especially first-time users—to set up a consultation with the core at the start of new projects to discuss your experimental goals and analysis requirements.