Currently the core offers two different nascent RNA library construction services: PRO-seq and TT-seq. Please see the links in the side-bar for detailed information about each of them and the table below for an overview of sample requirements.
|
Method |
Minimum # cells |
Requested # of cells |
Cell condition |
Spike-in available |
Spike-in required |
|---|---|---|---|---|---|
|
PRO-seq |
1 million |
1-3 million |
permeabilized frozen cells |
yes |
yes |
|
TT-seq |
5-8 million * |
5-8 million* |
4sU labeled cells in Trizol |
yes |
yes |
* Number of cells needed for TT-seq depends on cell type and labeling time. We request 100ug of total RNA.
If you are interested in differential expression analysis, we recommend preparing a minimum of biological duplicates of your samples. For other applications, more or fewer replicates may be appropriate. We encourage users—especially first-time users—to set up a consultation with the core at the start of new projects to discuss your experimental goals and analysis requirements.