@article {XU20221156,
	title = {Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis},
	journal = {Molecular Cell},
	volume = {82},
	number = {6},
	year = {2022},
	pages = {1156-1168.e7},
	abstract = {Summary N6-methyladenosine (m6A) methylation is co-transcriptionally deposited on mRNA, but a possible role of m6A on transcription remains poorly understood. Here, we demonstrate that the METTL3/METTL14/WTAP m6A methyltransferase complex (MTC) is localized to many promoters and enhancers and deposits the m6A modification on nascent transcripts, including pre-mRNAs, promoter upstream transcripts (PROMPTs), and enhancer RNAs. PRO-seq analyses demonstrate that nascent RNAs originating from both promoters and enhancers are significantly decreased in the METTL3-depleted cells. Furthermore, genes targeted by the Integrator complex for premature termination are depleted of METTL3, suggesting a potential antagonistic relationship between METTL3 and Integrator. Consistently, we found the Integrator complex component INTS11 elevated at promoters and enhancers upon loss of MTC or nuclear m6A binders. Taken together, our findings suggest that MTC-mediated m6A modification protects nascent RNAs from Integrator-mediated termination and promotes productive transcription, thus unraveling an unexpected layer of gene regulation imposed by RNA m6A modification.},
	keywords = {ALKBH5, Chromatin, enhancer, hnRNP G, INTS11, MA, METTL3, nascent RNA, promoter},
	issn = {1097-2765},
	doi = {https://doi.org/10.1016/j.molcel.2022.02.006},
	url = {https://www.sciencedirect.com/science/article/pii/S1097276522001113},
	author = {Wenqi Xu and Chenxi He and Emily G. Kaye and Jiahui Li and Mandi Mu and Nelson, Geoffrey M. and Li Dong and Jiahua Wang and Wu, Feizhen and Yujiang Geno Shi and Adelman, Karen and Lan, Fei and Shi, Yang and Hongjie Shen}
}
