#  Sample Guide 

 



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Currently the core offers two different nascent RNA library construction services: PRO-seq and TT-seq. Please see the links in the side-bar for detailed information about each of them and the table below for an overview of sample requirements.

Sort**Method****Minimum # cells**

**Requested # of cells**

**Cell condition**

**Spike-in available**

**Spike-in required**

PRO-seq



1 million



1-3 million



permeabilized frozen cells



yes



yes



TT-seq



5-8 million \*



5-8 million\*



4sU labeled cells in Trizol



yes



yes







*\* Number of cells needed for TT-seq depends on cell type and labeling time. We request 100ug of total RNA.*

If you are interested in differential expression analysis, we recommend preparing a minimum of biological duplicates of your samples. For other applications, more or fewer replicates may be appropriate. We encourage users—especially first-time users—to set up a consultation with the core at the start of new projects to discuss your experimental goals and analysis requirements.